thrS [2017-8-11 16:0:3]

threonyl-tRNA synthetase (major)

Locus: BSU28950

Isoelectric point: 5.21

Molecular weight: 73.34 kDa

Protein length: 643 aa

Gene length: 1929 bp

Function: translation

Product: threonyl-tRNA synthetase (major)

Essential: no

E.C.: 6.1.1.3

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → Aminoacyl-tRNA synthetases

Gene

Coordinates: 2,959,257 → 2,961,188

The protein

Catalyzed reaction/ biological activity

• ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr) (according to Swiss-Prot)

Protein family

• class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)

Paralogous protein(s)

• ThrZ, one of the two proteins has to be present for viability PubMed

Modification

• Cys573 is S-bacillithiolated by NaOCl stress PubMed

Structure

• 1TJE (from Escherichia coli, 44% identity, 63% similarity) PubMed

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed
• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: thrS
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • T-box: anti-termination, in T-box

  Regulation

  • expression transiently increases in the forespore PubMed
  • the T-box RNA is degraded by RNase Y PubMed

• Genes: dnaB-dnaI-ytxB-ytxC-thrS
  Description: PubMed

References

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